mmp13 (Proteintech)
Proteintech is a verified supplier 
96
Structured Review
Proteintech
mmp13
Mmp13, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 809 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mmp13/MMP13+Antibody/pmc12963906-78-33-35
Average 96 stars, based on 809 article reviews
Mmp13, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 809 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mmp13/MMP13+Antibody/pmc12963906-78-33-35
Average 96 stars, based on 809 article reviews
mmp13 - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "Collagen II hydrogel-mediated sustained delivery of lacosamide attenuates cartilage degeneration and pain in osteoarthritis"
Article Title: Collagen II hydrogel-mediated sustained delivery of lacosamide attenuates cartilage degeneration and pain in osteoarthritis
Journal: Bioactive Materials
doi: 10.1016/j.bioactmat.2026.02.045
Figure Legend Snippet: Non-selective Nav1.7 inhibitors regulate chondrocyte metabolism . (A) mRNA levels of COL2 and ACAN in primary human OA chondrocytes treated with 10 μM LCM, CBZ, or OXC for 24h. (B) mRNA levels of MMP13 and ADAMTS5 in primary human OA chondrocytes stimulated with 20 ng/mL IL-1β and 10 μM LCM, CBZ or OXC for 24h. (C, D) COL2 and ACAN (C) , MMP13 and ADAMTS5 (D) mRNA expression in human OA chondrocytes treated with various doses of LCM with/without IL-1β stimulated conditions. (E, F) mRNA levels of anabolic markers Col2 and Acan , as well as catabolic enzymes Mmp13 and Adamts5 , in primary chondrocytes isolated from WT and chondrocyte specific Nav1.7 knockout mice under basal conditions or following stimulation with 20 ng/mL IL-1β, with or without 10 μM LCM co-treatment for 24 h. (G) Representative immunofluorescence staining showing COL2 (green) and MMP13 (green) expression in C28/I2 chondrocytes treated with 20 ng/ml IL-1β and 10 nM LCM for 48h. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (H) Quantification of average optical density (AOD) shown in G. (I) Safranin O/Fast Green and IHC staining of human OA cartilage explants cultured with 20 ng/ml IL-1β and 10 nM LCM for 5 days. Scale bar = 100 μm. (J) Quantification of GAG area and OARSI scores in I. (K) Quantification of IHC staining in I. Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (A-F and H, J, K).
Techniques Used: Expressing, Isolation, Knock-Out, Immunofluorescence, Staining, Immunohistochemistry, Cell Culture, Comparison
Figure Legend Snippet: LCM regulated chondrocyte metabolism through enhancing HSP70 and midkine secretion . (A) ELISA quantification of midkine and HSP70 levels in conditioned medium (CM) collected from human OA chondrocytes treated with 10 nM LCM for 48 h. (B) mRNA levels of COL2 and ACAN in chondrocytes cultured with LCM- CM for 24 h. (C) mRNA levels of MMP13 and ADAMTS5 in human OA chondrocytes treated with 20 ng/mL IL-1β and LCM-CM for 24 h. (D) mRNA expression of COL2 and ACAN in chondrocytes treated with LCM-CM in the presence or absence of anti-HSP70 (αHSP70) antibody for 24 h. (E) mRNA expression of MMP13 and ADAMTS5 in human OA chondrocytes stimulated with 20 ng/ml IL-1β and LCM-CM in the presence or absence of anti-midkine (αMDK) antibody for 24 h. (F, G) Representative immunofluorescence staining of MMP13 (F) and COL2 (G) in C28/I2 chondrocytes with/without IL-1β, LCM-CM, anti-HSP70 antibody and anti-midkine antibody. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (H) Quantification of average optical density (AOD) of MMP13 and COL2 immunofluorescence shown in F and G. (I) Safranin O/Fast Green and IHC staining for MMP13 in human OA cartilage explants cultured with 20 ng/mL IL-1β in the presence or absence of LCM-CM or anti-midkine antibody for 5 days. Scale bar = 100 μm. (J) Safranin O/Fast Green and IHC staining for COL2 in cartilage explants cultured with LCM-CM with or without anti-HSP70 antibody for 5 days. Scale bar = 100 μm. (K) Quantification of GAG area and OARSI scores in I. (L) Quantification of IHC staining in I. (M) Quantification of GAG area and OARSI scores in J. (N) Quantification of IHC staining in J. Data are presented as mean ± SD. Statistical analysis: unpaired two-tailed Student's t-test (A, B) and one-way ANOVA with post hoc comparison (C-E and H, K-N).
Techniques Used: Enzyme-linked Immunosorbent Assay, Cell Culture, Expressing, Immunofluorescence, Staining, Immunohistochemistry, Two Tailed Test, Comparison
Figure Legend Snippet: Systemic administration of LCM attenuates OA progression and pain . (A) Schematic of the experimental outline. Twelve-week-old male WT mice underwent DMM surgery. Beginning 4 weeks post-surgery, mice were treated daily with either CBZ (10 mg/kg) or LCM (1, 10, or 50 mg/kg) by oral gavage for a total duration of 8 weeks. (n = 6). (B) Representative Safranin O/Fast Green stained images of knee joints at 12 weeks post-DMM surgery, systemically treated with CBZ (10 mg/kg/day) or LCM (1, 10, or 50 mg/kg/day) (n = 6). Scale bar = 100 μm. (C) Quantification of OARSI scores, subchondral bone plate (SBP) thickness, osteophyte formation, and synovitis. (D, E) Behavioral outcomes assessed by open-field travel distance (D) and von Frey test (E). (F) Representative IHC staining of Col2, Aggrecan neoepitope, Mmp13, and Adamts5 in joint sections (n = 6). Scale bar = 50 μm ∗Vehicle versus LCM (50 mg/kg/day), # Vehicle versus LCM (10 mg/kg/day),(∗ ,# P < 0.05; ∗∗ ,## P < 0.01). Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (C) and unpaired two-tailed Student's t-test (D, E).
Techniques Used: Staining, Immunohistochemistry, Comparison, Two Tailed Test
Figure Legend Snippet: Intra-articular delivery of LCM protects against OA . (A) Schematic of the experimental outline. Twelve-week-old male WT mice underwent DMM surgery. Beginning 4 weeks post-surgery, mice received intra-articular injections of CBZ (1 mg/kg, three times per week) or LCM (0.1 or 1 mg/kg, three times per week) for a total duration of 8 weeks (n = 6). (B) Representative Safranin O/Fast Green stained sections of knee joints at 12 weeks post-surgery, treated with or without CBZ (1 mg/kg, 3 × /week) or LCM (0.1 or 1 mg/kg, 3 × /week) intra-articularly (n = 6). Scale bar = 100 μm. (C) Quantification of OARSI scores, subchondral bone plate (SBP) thickness, osteophyte formation, and synovitis. (D, E) Behavioral outcomes assessed by open-field travel distance (D) and von Frey test (E). (F) Representative IHC staining of Col2, Aggrecan neoepitope, Mmp13, and Adamts5 in joint sections (n = 6). Scale bar = 50 μm ∗Vehicle versus LCM (1 mg/kg, 3 × /week), # Vehicle versus LCM (0.1 mg/kg, 3 × /week),(∗ ,# P < 0.05). Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (C) and unpaired two-tailed Student's t-test (D, E).
Techniques Used: Staining, Immunohistochemistry, Comparison, Two Tailed Test
Figure Legend Snippet: Hydrogel-mediated delivery of LCM provides prolonged joint retention and therapeutic efficacy . (A) Schematic of the experimental outline. Twelve-week-old male WT mice underwent DMM surgery. Beginning at 4 weeks post-surgery, mice received intra-articular injections of either blank hydrogel or LCM-loaded hydrogel once monthly for 2 months (n = 6). (B) Safranin O/Fast Green–stained sections of knee joints 12 weeks post-DMM surgery, following intra-articular injection of LCM-loaded hydrogel (n = 6). Scale bar = 100 μm. (C) Quantification of OARSI scores, SBP thickness, osteophyte size, and synovitis in each group. (D, E) Behavior test outcomes assessed by open-field movement (D) and von Frey test (E). (F) Representative IHC staining of Col2, Aggrecan neoepitope, Mmp13, and Adamts5 in joint sections (n = 6). Scale bar = 50 μm. (G) Quantification of IHC-positive staining in (F). ∗Vehicle versus LCM-loaded hydrogel, (∗ P < 0.05; ∗∗ P < 0.01, ∗∗∗ P < 0.001). Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (C, G) and unpaired two-tailed Student's t-test (D, E).
Techniques Used: Drug discovery, Staining, Injection, Immunohistochemistry, Comparison, Two Tailed Test
Related Articles
Incubation:Article Title: Therapeutic effects of Tetramethylpyrazine on Cartilage in Rat Model of Post-traumatic Osteoarthritis Article Snippet: .. The sections were then incubated with the following primary antibodies: anti- ADAMTS5 (1:100, BS-3573R, Biosharp Co.,Ltd, Beijing, China), anti-COL II (1:100, ab34712, Abcam, Cambridge, MA, USA), and Article Title: HDAC6 exacerbates osteoarthritis by promoting macrophage inflammation-mediated chondrocyte degeneration. Article Snippet: Osteoarthritis (OA) is a degenerative joint disorder characterized by cartilage degradation and synovial inflammation, with its pathogenesis involving intricate interactions between cellular metabolic processes and inflammatory pathways.. In this study, we established OA models in both wild-type and histone deacetylase 6 (HDAC6) knockout mice via anterior cruciate ligament transection (ACLT) to investigate the role of HDAC6 in OA progression.. Histological and immunohistochemical analyses revealed that the absence of HDAC6 significantly mitigated ACLT-induced cartilage damage and synovitis in mice, while also restoring imbalances in cartilage metabolic markers. Article Title: Injectable microgels loaded with programmable WELNs based on mendelian randomization macroanalysis alleviate osteoarthritis by restoring the circadian rhythm Article Snippet: In Western blot analysis, the total protein was extracted from different groups using RIPA lysis buffer (Beyotime), Protein concentration was detected by microplate reader and an equal mass of protein (20 μg) was separated by SDS-PAGE (Beyotime), which were transferred to nitrocellulose (NC) membranes (Millipore). .. The membranes were blocked and incubated with primary antibodies including Article Title: Sequential delivery of sinigrin and dabigatran by an in situ self-stabilizing dynamic hydrogel attenuates intervertebral disc degeneration Article Snippet: Prior to SDS-PAGE, protein concentrations were quantified using a BCA Protein Assay Kit (Beyotime, China). .. The membranes were then incubated overnight with anti-GAPDH (1:50 000, AC054, abclone, China), anti-Col2α1(1:500, A19308, abclone, China), anti-Acan (1:1000, A8536, abclone, China), anti-SOX9 (1:1000, A19710, abclone, China), Article Title: Therapeutic effects of Tetramethylpyrazine on Cartilage in Rat Model of Post-traumatic Osteoarthritis Article Snippet: .. The sections were then incubated with the following primary antibodies: anti- ADAMTS5 (1:100, BS-3573R, Biosharp Co.,Ltd, Beijing, China), anti-COL II (1:100, ab34712, Abcam, Cambridge, MA, USA), and Article Title: Injectable microgels carrying engineered biomimetic nanoparticles for osteoarthritis therapy via dual-targeted senescent chondrocyte clearance and endogenous repair promotion Article Snippet: Inter-rater reliability was evaluated using the intraclass correlation coefficient (ICC), calculated based on a two-way random-effects model. For immunohistochemistry, antigen retrieval was conducted using EDTA Antigen Retrieval Solution (Solarbio, China) at 55 °C for 8 h. Endogenous peroxidase activity was blocked with 3 % H 2 O 2 for 10 min, followed by 1-h blocking in PBS containing 20 % donkey serum and 0.3 % Triton X-100. .. Then, sections were incubated overnight at 4 °C with primary antibodies: anti-COL2A1 (28459-1-AP, 1:200, Proteintech), Article Title: Injectable microgels loaded with programmable WELNs based on mendelian randomization macroanalysis alleviate osteoarthritis by restoring the circadian rhythm Article Snippet: .. Fixed chondrocytes and sections which obtained from articular cartilage and explants were permeabilized with Triton X-100 (Beyotime) for 8 min. After incubating with blocking buffer (3 % BSA solution), the sections and chondrocytes were incubated with primary antibodies including Staining:Article Title: A scaffold-driven coordination paradigm with built-in hypoxia mimicry and hypertrophy inhibition for functional cartilage regeneration Article Snippet: Hypoxia-mimicking microenvironment represents a promising solution in promoting chondrogenic differentiation of stem cells for cartilage repair.. However, many existing methods exhibit inadequate bioavailability and risk of toxic accumulation, and the prolonged low oxygen condition can further induce chondrocyte hypertrophy, ultimately compromising the repair process.. To overcome these challenges, this study introduces a materialdriven paradigm to design a bioactive hypoxia-mimetic hydrogel, PACG-GEL-KGN (PGK), featuring robust mechanical strength, hypoxic inducing activity, and hypertrophy inhibition effect. Membrane:Article Title: Sequential delivery of sinigrin and dabigatran by an in situ self-stabilizing dynamic hydrogel attenuates intervertebral disc degeneration Article Snippet: Prior to SDS-PAGE, protein concentrations were quantified using a BCA Protein Assay Kit (Beyotime, China). .. The membranes were then incubated overnight with anti-GAPDH (1:50 000, AC054, abclone, China), anti-Col2α1(1:500, A19308, abclone, China), anti-Acan (1:1000, A8536, abclone, China), anti-SOX9 (1:1000, A19710, abclone, China), Blocking Assay:Article Title: Injectable microgels loaded with programmable WELNs based on mendelian randomization macroanalysis alleviate osteoarthritis by restoring the circadian rhythm Article Snippet: .. Fixed chondrocytes and sections which obtained from articular cartilage and explants were permeabilized with Triton X-100 (Beyotime) for 8 min. After incubating with blocking buffer (3 % BSA solution), the sections and chondrocytes were incubated with primary antibodies including |
![TMH/QG+NIR attenuates ferroptosis and matrix degradation in TBHP‐treated AFCs. (A) Schematic illustration of AF cells co‐culture system. (B) DCFH‐DA fluorescence staining images and quantitative analysis of TBHP‐pretreated AFCs after different treatments. Scale bar: 50 µm. (C) Intracellular ROS levels in TBHP‐pretreated AFCs measured by flow cytometry analysis and quantitative results. (D,E) Quantitative analysis of MDA and GSH levels in TBHP‐pretreated AFCs co‐cultured with QG, TMH and TMH/QG MN (with NIR irradiation). (F,G) Representative images and quantification of labile iron pool by FerroOrange staining and lipid peroxidation by C11‐BODIPY staining in TBHP‐pretreated AFCs co‐culture with QG, TMH and TMH/QG MN (with NIR irradiation). Scale bar: 20 µm. (H) Western blot analysis and corresponding quantitative statistics of extracellular matrix (COL1), remodeling markers (MMP3, <t>MMP13),</t> and ferroptosis‐related proteins (ACSL4, GPX4). The corresponding uncropped blot images are provided in Table . (I) Immunofluorescence staining and fluorescence intensity quantification of COL1 and MMP3 expression in TBHP‐pretreated AFCs co‐cultured with QG, TMH, and TMH/QG MN (with NIR irradiation). Scale bar: 20 µm. Data are presented as mean ± SD (n ≥ 3), and p ‐values were calculated using one‐way ANOVA with Tukey's multiple comparisons test [(B) to (I)].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6280/pmc13616280/pmc13616280__ADVS-9999-e77829-g005.webp.jpg)
![DNMT3A‐mediated ATF3 promoter methylation underlies the anti‐ferroptotic effect of TMH/QG+NIR in degenerative AFCs. (A) GSEA plots identify significant enrichment of DEGs in the DNA methylation pathway. (B,C) Methylation‐specific PCR and corresponding quantification of the Atf3 promoter methylation level in degenerative AFCs treated with the TMH/QG+NIR microneedle system. The corresponding uncropped gel images are provided in Table . (D) Western blot analysis of DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B) in the TBHP+NIR and TBHP+NIR+TMH/QG groups. The corresponding uncropped blot images are provided in Table . (E) ChIP‐qPCR analysis of DNMT1, DNMT3A, and DNMT3B enrichment at the Atf3 promoter region. (F) ChIP‐qPCR analysis specifically quantifying DNMT3A enrichment at the Atf3 promoter. (G) Measurement of MDA content. (H) Western blot analysis of SLC7A11 and ATF3 protein levels in the TBHP+NIR+TMH/QG group with or without DY‐46‐2. The corresponding uncropped blot images are provided in Table . (I) Relative mRNA expression level of Slc7a11 and Atf3 in the TBHP+NIR+TMH/QG group with or without the DNMT3A inhibitor DY‐46‐2. (J) Flow cytometry analysis of intracellular ROS levels. (K) Representative FerroOrange staining images in the TBHP+NIR+TMH/QG group with or without DY‐46‐2. Scale bar: 20 µm. (L) Western blot analysis of key ferroptosis (ACSL4 and GPX4) and extracellular matrix (COL1, MMP3 and MMP13) markers in the TBHP+NIR+TMH/QG group with or without DY‐46‐2. The corresponding uncropped blot images are provided in Table . (M) IHC staining of ATF3, SLC7A11 and DNMT3A expression in human disc tissues from mild and severe degeneration groups. Scale bar: 20 µm. (N) MSP of the ATF3 promoter methylation level in human mild and severe degeneration groups. The corresponding uncropped gel images are provided in Table . (O) Western blot analysis of ATF3, SLC7A11 and DNMT3A protein expression in human disc tissues from mild and severe degeneration groups. The corresponding uncropped blot images are provided in Table . (P) Relative mRNA expression of ATF3 in human mild and severe degeneration groups. (Q–S) Correlation analysis of relative ATF3 expression in AF tissue and relative GPX4 , SLC7A11 and DNMT3A expression. (T) Schematic model illustrating the proposed mechanism. Data are presented as mean ± SD (n ≥ 3), and p ‐values were calculated using two‐tailed Student's t ‐test [(C), (F), (G), (I) and (P)] and one‐way ANOVA with Tukey's multiple comparisons test (E).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6280/pmc13616280/pmc13616280__ADVS-9999-e77829-g006.webp.jpg)